The Preparation of Steroid N-Hydroxysuccinimide Esters and Their Reactivities with Bovine Serum albumin1)

Hiroshi Hosoda, Yasuo Sakai, Hiroko Yoshida, Shinichi Miyairi, Kazuo Ishii, Toshio Nambara

研究成果: Article査読

82 被引用数 (Scopus)

抄録

The N-hydroxysuccinimide esters of testosterone and cortisol were prepared from the corresponding carboxylic acid derivatives by a carbodiimide method. These activated esters were covalently linked to bovine serum albumin in organic solvent/phosphate buffer (pH 7.4) employing various molar ratios of the steroid to protein. The results indicated that the activated ester method would be useful for enzyme labeling in enzyme immunoassay of steroids in respects of simplicity and reproducibility.

本文言語English
ページ(範囲)742-746
ページ数5
ジャーナルChemical and Pharmaceutical Bulletin
27
3
DOI
出版ステータスPublished - 1979

ASJC Scopus subject areas

  • 化学 (全般)
  • 創薬

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