The preparation of amperometric xanthine sensors based on multilayer thin films containing xanthine oxidase

Tomonori Hoshi, Takio Noguchi, Jun Ichi Anzai

研究成果: Article査読

20 被引用数 (Scopus)

抄録

An amperometric xanthine biosensor was prepared by immobilizing xanthine oxidase (XOx) on the surface of a platinum (Pt) disk electrode. The Pt electrode was first covered with a polyelectrolyte multilayer (PEM) film composed of poly(allylamine hydrochloride) (PAH) and poly(vinyl sulfate) (PVS) to block the Pt surface from the access of uric acid, a reaction product of the XOx-catalyzed oxidation reaction of xanthine, because uric acid can be electrochemically oxidized on the Pt electrode to induce interference. The PEM film-covered Pt electrode was further modified with a XOx-containing multilayer film composed of XOx and poly(dimethyldiallylammonium chloride) (PDDA). The xanthine biosensors thus prepared can be used successfully for detecting xanthine in the concentration range of 3-300 μM. The output current of the sensors depended on the number of the XOx/PDDA layers in the film. The PAH/PVS layer was effective to eliminate the uric acid interference.

本文言語English
ページ(範囲)100-103
ページ数4
ジャーナルMaterials Science and Engineering C
26
1
DOI
出版ステータスPublished - 2006 1月

ASJC Scopus subject areas

  • 材料科学(全般)
  • 凝縮系物理学
  • 材料力学
  • 機械工学

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