The PDR-type ABC transporters AtrA and AtrG are involved in azole drug resistance in aspergillus oryzae

Daisuke Miura, Kohei Sugiyama, Atsushi Ito, Ayumi Ohba-Tanaka, Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi

研究成果: Article査読

6 被引用数 (Scopus)

抄録

For strain improvement of Aspergillus oryzae, development of the transformation system is essential, wherein dominant selectable markers, including drug-resistant genes, are available. However, A. oryzae generally has a relatively high resistance to many antifungal drugs effective against yeasts and other filamentous fungi. In the course of the study, while investigating azole drug resistance in A. oryzae, we isolated a spontaneous mutant that exhibited high resistance to azole fungicides and found that pleiotropic drug resistance (PDR)-type ATP-binding cassette (ABC) transporter genes were upregulated in the mutant; their overexpression in the wild-type strain increased azole drug resistance. While deletion of the gene designated atrG resulted in increased azole susceptibility, double deletion of atrG and another gene (atrA) resulted in further azole hypersensitivity. Overall, these results indicate that the ABC transporters AtrA and AtrG are involved in azole drug resistance in A. oryzae.

本文言語English
ページ(範囲)1840-1848
ページ数9
ジャーナルBioscience, Biotechnology and Biochemistry
82
10
DOI
出版ステータスPublished - 2018

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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