The parasitophorous vacuole membrane of toxoplasma gondii is targeted for disruption by ubiquitin-like conjugation systems of autophagy

Jayoung Choi, Sunmin Park, Scott B. Biering, Elizabeth Selleck, Catherine Y. Liu, Xin Zhang, Naonobu Fujita, Tatsuya Saitoh, Shizuo Akira, Tamotsu Yoshimori, L. David Sibley, Seungmin Hwang, Herbert W. Virgin

    研究成果: Article査読

    136 被引用数 (Scopus)

    抄録

    Autophagy is a lysosomal degradation pathway that is important in cellular homeostasis. Prior work showed a key role for the autophagy related 5 (Atg5) in resistance to Toxoplasma gondii. Here we show that the cassette of autophagy proteins involved in the conjugation of microtubule-associated protein 1 light chain 3 (LC3) to phosphatidylethanolamine, including Atg7, Atg3, and the Atg12-Atg5-Atg16L1 complex play crucial roles in the control of T.gondii invitro and invivo. In contrast, pharmacologic modulation of thedegradative autophagy pathway or genetic deletion of other essential autophagy genes had no substantial effects. Rather the conjugation system was required for targeting of LC3 and interferon-γ effectors ontothe vacuolar membrane of T.gondii and its consequent disruption. These data suggest that the ubiquitin-like conjugation systems that reorganize intracellular membranes during canonical autophagy are necessary for proper targeting of immune effectors to the intracellular vacuole membranes utilized by pathogens.

    本文言語English
    ページ(範囲)924-935
    ページ数12
    ジャーナルImmunity
    40
    6
    DOI
    出版ステータスPublished - 2014 6月 19

    ASJC Scopus subject areas

    • 免疫アレルギー学
    • 免疫学
    • 感染症

    フィンガープリント

    「The parasitophorous vacuole membrane of toxoplasma gondii is targeted for disruption by ubiquitin-like conjugation systems of autophagy」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

    引用スタイル