The N-glycolyl form of mouse sialyl Lewis X is recognized by selectins but not by HECA-452 and FH6 antibodies that were raised against human cells

Junya Mitoma, Tatsuo Miyazaki, Mark Sutton-Smith, Misa Suzuki, Hideo Saito, Jiunn Chern Yeh, Takehiro Kawano, Ole Hindsgaul, Peter H. Seeberger, Maria Panico, Stuart M. Haslam, Howard R. Morris, Richard D. Cummings, Anne Dell, Minoru Fukuda

研究成果: Article査読

25 被引用数 (Scopus)

抄録

E-, P- and L-selectins critically function in lymphocyte recirculation and recruiting leukocytes to inflammatory sites. MECA-79 antibody inhibits L-selectin-mediated lymphocyte adhesion in several species and does not require sialic acid in its epitope. Many other antibodies, however, recognize human selectin ligands expressing N-acetylneuraminic acid but not mouse selectin ligands expressing N-glycolylneuraminic acid, suggesting that difference in sialic acid in sialyl Lewis X leads to differential reactivity. We found that HECA-452 and FH6 monoclonal antibodies bind Chinese hamster ovary (CHO) cells expressing N-acetylneuraminyl Lewis X oligosaccharide but not its N-glycolyl form. Moreover, synthetic N-acetylneuraminyl Lewis X oligosaccharide but not its N-glycolyl oligosaccharide inhibited HECA-452 and FH6 binding. By contrast, E-, P- and L-selectin bound to CHO cells regardless of whether they express N-acetyl or N-glycolyl form of sialyl Lewis X, showing that selectins have a broader recognition capacity than HECA-452 and FH-6 anti-sialyl Lewis x antibodies.

本文言語English
ページ(範囲)511-523
ページ数13
ジャーナルGlycoconjugate Journal
26
5
DOI
出版ステータスPublished - 2009 7月
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

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