The N-glycolyl form of mouse sialyl Lewis X is recognized by selectins but not by HECA-452 and FH6 antibodies that were raised against human cells

Junya Mitoma, Tatsuo Miyazaki, Mark Sutton-Smith, Misa Suzuki, Hideo Saito, Jiunn Chern Yeh, Takehiro Kawano, Ole Hindsgaul, Peter H. Seeberger, Maria Panico, Stuart M. Haslam, Howard R. Morris, Richard D. Cummings, Anne Dell, Minoru Fukuda

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    E-, P- and L-selectins critically function in lymphocyte recirculation and recruiting leukocytes to inflammatory sites. MECA-79 antibody inhibits L-selectin-mediated lymphocyte adhesion in several species and does not require sialic acid in its epitope. Many other antibodies, however, recognize human selectin ligands expressing N-acetylneuraminic acid but not mouse selectin ligands expressing N-glycolylneuraminic acid, suggesting that difference in sialic acid in sialyl Lewis X leads to differential reactivity. We found that HECA-452 and FH6 monoclonal antibodies bind Chinese hamster ovary (CHO) cells expressing N-acetylneuraminyl Lewis X oligosaccharide but not its N-glycolyl form. Moreover, synthetic N-acetylneuraminyl Lewis X oligosaccharide but not its N-glycolyl oligosaccharide inhibited HECA-452 and FH6 binding. By contrast, E-, P- and L-selectin bound to CHO cells regardless of whether they express N-acetyl or N-glycolyl form of sialyl Lewis X, showing that selectins have a broader recognition capacity than HECA-452 and FH-6 anti-sialyl Lewis x antibodies.

    元の言語English
    ページ(範囲)511-523
    ページ数13
    ジャーナルGlycoconjugate Journal
    26
    発行部数5
    DOI
    出版物ステータスPublished - 2009 7 1

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    フィンガープリント The N-glycolyl form of mouse sialyl Lewis X is recognized by selectins but not by HECA-452 and FH6 antibodies that were raised against human cells' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Mitoma, J., Miyazaki, T., Sutton-Smith, M., Suzuki, M., Saito, H., Yeh, J. C., Kawano, T., Hindsgaul, O., Seeberger, P. H., Panico, M., Haslam, S. M., Morris, H. R., Cummings, R. D., Dell, A., & Fukuda, M. (2009). The N-glycolyl form of mouse sialyl Lewis X is recognized by selectins but not by HECA-452 and FH6 antibodies that were raised against human cells. Glycoconjugate Journal, 26(5), 511-523. https://doi.org/10.1007/s10719-008-9207-8