The MMP activity in developing rat molar roots and incisors demonstrated by in situ zymography

Ichiro Sakuraba, Junko Hatakeyama, Yuji Hatakeyama, Ichiro Takahashi, Hideaki Mayanagi, Yasuyuki Sasano

研究成果: Article査読

18 被引用数 (Scopus)

抄録

Matrix metalloproteinases (MMPs) have been expressed during root development and periodontal tissue formation, whereas it is not known if these MMP molecules are enzymatically active to degrade the extracellular matrices (ECMs). The present study was designed to investigate the gelatinolytic and collagenolytic activity in rat molar root and incisor development. Three-week old rat mandibles were frozen and cut without fixation or decalcification and processed for in situ zymography using substrates gelatin and collagen. The enzymatic activity was assessed according to the intensity of fluorescence due to the lysis of the substrates. Odontoblasts, predentin, cementum, bone and the enamel matrix showed the high activity. The present study demonstrated MMP activity in calcified tissues using in situ zymography for the first time and the possible involvement of the MMP activity in molar root and incisor development and periodontal tissue formation.

本文言語English
ページ(範囲)87-93
ページ数7
ジャーナルJournal of Molecular Histology
37
1-2
DOI
出版ステータスPublished - 2006 1

ASJC Scopus subject areas

  • Histology
  • Physiology
  • Cell Biology

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