The majority of early primordial germ cells acquire pluripotency by AKT activation

Yasuhisa Matsui, Asuka Takehara, Yuko Tokitake, Makiko Ikeda, Yuka Obara, Yuiko Morita-Fujimura, Tohru Kimura, Toru Nakano

研究成果: Article査読

15 被引用数 (Scopus)

抄録

Primordial germ cells (PGCs) are undifferentiated germ cells in embryos, the fate of which is to become gametes; however, mouse PGCs can easily be reprogrammed into pluripotent embryonic germ cells (EGCs) in culture in the presence of particular extracellular factors, such as combinations of Steel factor (KITL), LIF and bFGF (FGF2). Early PGCs form EGCs more readily than do later PGCs, and PGCs lose the ability to form EGCs by embryonic day (E) 15.5. Here, we examined the effects of activation of the serine/threonine kinase AKT in PGCs during EGC formation; notably, AKT activation, in combination with LIF and bFGF, enhanced EGC formation and caused ∼60% of E10.5 PGCs to become EGCs. The results indicate that the majority of PGCs at E10.5 could acquire pluripotency with an activated AKT signaling pathway. Importantly, AKT activation did not fully substitute for bFGF and LIF, and AKT activation without both LIF and bFGF did not result in EGC formation. These findings indicate that AKT signal enhances and/or collaborates with signaling pathways of bFGF and of LIF in PGCs for the acquisition of pluripotency.

本文言語English
ページ(範囲)4457-4467
ページ数11
ジャーナルDevelopment (Cambridge)
141
23
DOI
出版ステータスPublished - 2014 12 1

ASJC Scopus subject areas

  • 分子生物学
  • 発生生物学

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