@article{4976f8aa855047a98edd5148c0ecc498,
title = "The Kr{\"u}ppel-like factor KLF15 inhibits transcription of the adrenomedullin gene in adipocytes",
abstract = "KLF15 (Kr{\"u}ppel-like factor 15) plays a key role in adipocyte differentiation and glucose transport in adipocytes through activation of its target genes. We have now identified six target genes regulated directly by KLF15 in 3T3-L1 mouse adipocytes with the use of a combination of microarray-based chromatin immunoprecipitation and gene expression analyses. We confirmed the direct regulation by KLF15 of one of these genes, that for adrenomedullin, with the use of a luciferase reporter assay in 3T3-L1 preadipocytes and adipocytes. Such analysis revealed that the most proximal CACCC element in the promoter of the human adrenomedullin gene (located in the region spanning nucleotides -70 and -29) is required for trans-inhibition by KLF15. Furthermore, chromatin immunoprecipitation showed that KLF15 binds to this region of the human adrenomedullin gene promoter in cultured human adipocytes. These results thus implicate KLF15 in the regulation of adrenomedullin expression in adipose tissue.",
keywords = "Adipocyte, Adrenomedullin, ChIP-chip, KLF15, Microarray, Transcription",
author = "Tomoki Nagare and Hiroshi Sakaue and Mototsugu Takashima and Kazuhiro Takahashi and Hideyuki Gomi and Yasushi Matsuki and Eijiro Watanabe and Ryuji Hiramatsu and Wataru Ogawa and Masato Kasuga",
note = "Funding Information: This work was supported by a grant from Takeda Science Foundation to H.S.; a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) to H.S. and M.K.; a grant for the 21st Century COE Program “Center of Excellence for Signal Transduction Disease: Diabetes Mellitus as Model” from MEXT to M.K.; a grant for the Cooperative Link of Unique Science and Technology for Economy Revitalization (CLUSTER) from MEXT to M.K.; Grants-in-Aid for Scientific Research (C) and for Creative Scientific Research from the Japan Society for the Promotion of Science (JSPS) to H.S.; and a Grant-in-Aid for Creative Scientific Research from JSPS to M.K. ",
year = "2009",
month = jan,
day = "30",
doi = "10.1016/j.bbrc.2008.12.020",
language = "English",
volume = "379",
pages = "98--103",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",
}