The Krüppel-like factor KLF15 inhibits transcription of the adrenomedullin gene in adipocytes

Tomoki Nagare, Hiroshi Sakaue, Mototsugu Takashima, Kazuhiro Takahashi, Hideyuki Gomi, Yasushi Matsuki, Eijiro Watanabe, Ryuji Hiramatsu, Wataru Ogawa, Masato Kasuga

研究成果: Article査読

12 被引用数 (Scopus)

抄録

KLF15 (Krüppel-like factor 15) plays a key role in adipocyte differentiation and glucose transport in adipocytes through activation of its target genes. We have now identified six target genes regulated directly by KLF15 in 3T3-L1 mouse adipocytes with the use of a combination of microarray-based chromatin immunoprecipitation and gene expression analyses. We confirmed the direct regulation by KLF15 of one of these genes, that for adrenomedullin, with the use of a luciferase reporter assay in 3T3-L1 preadipocytes and adipocytes. Such analysis revealed that the most proximal CACCC element in the promoter of the human adrenomedullin gene (located in the region spanning nucleotides -70 and -29) is required for trans-inhibition by KLF15. Furthermore, chromatin immunoprecipitation showed that KLF15 binds to this region of the human adrenomedullin gene promoter in cultured human adipocytes. These results thus implicate KLF15 in the regulation of adrenomedullin expression in adipose tissue.

本文言語English
ページ(範囲)98-103
ページ数6
ジャーナルBiochemical and biophysical research communications
379
1
DOI
出版ステータスPublished - 2009 1 30

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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