TY - JOUR
T1 - The isolation and characterization of a novel cDNA demonstrating an altered mRNA level in nontumorigenic wilms' microcell hybrid cells
AU - Dowdy, Steven F.
AU - Lai, Kin man
AU - Weissman, Bernard E.
AU - Matsui, Yasuhisa
AU - Hogan, Brigid L.M.
AU - Stanbridge, Eric J.
N1 - Funding Information:
The evolutionary Southern blot was kindly provided by B.Zbar (National Cancer Institute, Bethesda, MD) and human-hamster mapping panel by J.Wasmuth (UC, Irvine, CA). We also thank K.Saito for excellent discussions. This work was supported in part by grants from the NIH CA 19104 (E.J.S), CA 44470 (B.E.W.), and CA 48799 (B.L.M.H.); and The Council For Tobacco Research, USA inc. 1475BR2 (E.J.S.). S.F.D. was supported by a predoctoral human genetics training grant from the University of California.
PY - 1991/10/25
Y1 - 1991/10/25
N2 - Wilms' tumor, a pediatric nephroblastoma, has been associated with genetic alterations of the 11p13 and 11p15 regions. The introduction of a der(11) chromosome into the G401 Wilms' tumor cell line has been shown previously to revert the tumorigenic phenotype. A subtractive cDNA/RNA hybridization performed between the tumorigenic parent (G401) and a nontumorigenic microcell hybrid of G401 (110.1/G401.1) containing the der(11) chromosome resulted in the identification of a single novel cDNA clone, designated QM. The cDNA is 745 nucleotides in length and encodes a predicted hydrophilic 25 kd basic protein, primarily consisting of alpha helices. The QM transcript is expressed in a wide variety of embryonic and adult tissues and demonstrates a down regulation of expression in adult kidney and heart. QM is also a member of a multigene family members of which map to chromosomes 6 and 14. The QM mRNA level is modulated between the tumorigenic and nontumorigenic cell lines and therefore may be involved in the maintenance of the nontumorigenic phenotype.
AB - Wilms' tumor, a pediatric nephroblastoma, has been associated with genetic alterations of the 11p13 and 11p15 regions. The introduction of a der(11) chromosome into the G401 Wilms' tumor cell line has been shown previously to revert the tumorigenic phenotype. A subtractive cDNA/RNA hybridization performed between the tumorigenic parent (G401) and a nontumorigenic microcell hybrid of G401 (110.1/G401.1) containing the der(11) chromosome resulted in the identification of a single novel cDNA clone, designated QM. The cDNA is 745 nucleotides in length and encodes a predicted hydrophilic 25 kd basic protein, primarily consisting of alpha helices. The QM transcript is expressed in a wide variety of embryonic and adult tissues and demonstrates a down regulation of expression in adult kidney and heart. QM is also a member of a multigene family members of which map to chromosomes 6 and 14. The QM mRNA level is modulated between the tumorigenic and nontumorigenic cell lines and therefore may be involved in the maintenance of the nontumorigenic phenotype.
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U2 - 10.1093/nar/19.20.5763
DO - 10.1093/nar/19.20.5763
M3 - Article
C2 - 1658743
AN - SCOPUS:0026048057
VL - 19
SP - 5763
EP - 5769
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 20
ER -