The human placenta expresses CYP17 and generates androgens de novo

J. C. Escobar, S. S. Patel, V. E. Beshay, T. Suzuki, Bruce R. Carr

研究成果: Article査読

46 被引用数 (Scopus)

抄録

Introduction: The human placenta is believed to have insignificant CYP17 expression, rendering it dependent on the maternal and fetal compartments for the necessary androgenic precursors to yield the high levels of estrogens seen in pregnancy. Objective: The aim of the study was to analyze whether the human trophoblast is capable of expressing CYP17 and producing androgens de novo. Methods: Human trophoblasts from fresh placentas and JEG-3 cells were used for all experiments. CYP17 mRNA analysis was performed via RT-PCR, and protein detection by Western blot and immunohistochemical staining. Steroid products were quantified using RIAs. Results: CYP17 mRNA was expressed in both cell types. CYP17 protein was detected by Western blotting and localized by immunostaining mainly to the cytoplasm of syncytiotrophoblasts. Measurement of 17α-hydroxyprogesterone, androstenedione, and their aromatized products in the media further demonstrated CYP17 expression and activity in the human trophoblast. Baseline levels of CYP17 steroid products were higher in primary cells and significantly increased in the presence of 22-hydroxycholesterol. Conclusions: We have demonstrated CYP17 mRNA and protein expression and activity in human trophoblasts. Considering the precursor concentration, blood flow, and mass of the placenta, we suggest that its contribution of androgens is an important source of estrogen production in pregnancy.

本文言語English
ページ(範囲)1385-1392
ページ数8
ジャーナルJournal of Clinical Endocrinology and Metabolism
96
5
DOI
出版ステータスPublished - 2011 5

ASJC Scopus subject areas

  • 内分泌学、糖尿病および代謝内科学
  • 生化学
  • 内分泌学
  • 臨床生化学
  • 生化学、医学

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