The HKM gene, which is identical to the MS1 gene of Arabidopsis thaliana, is essential for primexine formation and exine pattern formation

Tohru Ariizumi, Katsunori Hatakeyama, Kokichi Hinata, Shusei Sato, Tomohiko Kato, Satoshi Tabata, Kinya Toriyama

研究成果: Article査読

46 被引用数 (Scopus)

抄録

A male-sterile mutant of Arabidopsis thaliana was isolated by T-DNA tagging screening. Using transmission electron microscopy analysis, we revealed that the microspores of this mutant did not have normal thick primexine on the microspore at the tetrad stage. Instead, a moderately electron-dense layer formed around the microspores. Although microspores without normal primexine failed to form a proper reticulate exine pattern at later stages, sporopollenin was deposited and an exine-like hackly structure was observed on the microspores during the microspore stage. Thus, this mutant was named hackly microspore (hkm). It is speculated that the moderately electron-dense layer was primexine, which partially played its role in sporopollenin deposition onto the microspore. Cytological analysis revealed that the tapetum of the hkm mutant was significantly vacuolated, and that vacuolated tapetal cells crushed the microspores, resulting in the absence of pollen grains within the anther at anthesis. Single nucleotide polymorphism analysis demonstrated that the hkm mutation exists within the MS1 gene, which has been reportedly expressed within the tapetum. Our results suggest that the critical process of primexine formation is under sporophytic control.

本文言語English
ページ(範囲)1-7
ページ数7
ジャーナルSexual Plant Reproduction
18
1
DOI
出版ステータスPublished - 2005 6

ASJC Scopus subject areas

  • 植物科学
  • 細胞生物学

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