Background: A GTPase-deficient Rab27A(Q78L) mutation caused perinuclear melanosome aggregation by an unknown mechanism. Results: Forcible targeting of Rab27A(Q78L) to melanosomes by a novel melanosome-targeting tag restored peripheral melanosome distribution in Rab27A-deficient cells. Conclusion: The GTPase activity of Rab27A is required for its melanosome localization, not for melanosome transport. Significance: These findings provide new insights into the mechanism underlying the spatiotemporal regulation of Rab27A activity.
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