The exocytotic trafficking of TC10 occurs through both classical and nonclassical secretory transport pathways in 3T3L1 adipocytes

Robert T. Watson, Megumi Furukawa, Shian Huey Chiang, Diana Boeglin, Makoto Kanzaki, Alan R. Saltiel, Jeffrey E. Pessin

研究成果: Article査読

38 被引用数 (Scopus)

抄録

To examine the structural determinants necessary for TC10 trafficking, localization, and function in adipocytes, we generated a series of point mutations in the carboxyl-terminal targeting domain of TC10. Wild-type TC10 (TC10/WT) localized to secretory membrane compartments and caveolin-positive lipid raft microdomains at the plasma membrane. Expression of a TC10/C206S point mutant resulted in a trafficking and localization pattern that was indistinguishable from that of TC10/WT. In contrast, although TC10/C209S or the double TC10/C206,209S mutant was plasma membrane localized, it was excluded from both the secretory membrane system and the lipid raft compartments. Surprisingly, inhibition of Golgi membrane transport with brefeldin A did not prevent plasma membrane localization of TC10 or H-Ras. Moreover, inhibition of transGolgi network exit with a 19°C temperature block did not prevent the trafficking of TC10 or H-Ras to the plasma membrane. These data demonstrate that TC10 and H-Ras can both traffic to the plasma membrane by at least two distinct transport mechanisms in adipocytes, one dependent upon intracellular membrane transport and another independent of the classical secretory membrane system. Moreover, the transport through the secretory pathway is necessary for the localization of TC10 to lipid raft microdomains at the plasma membrane.

本文言語English
ページ(範囲)961-974
ページ数14
ジャーナルMolecular and cellular biology
23
3
DOI
出版ステータスPublished - 2003 2月
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 細胞生物学

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