The development of a staggered parallel separation liquid chromatography/tandem mass spectrometry system with on-line extraction for high-throughout screening of drug candidates in biological fluids

Junichi Goto, Masanori Nagata, Nariyasu Mano, Norihiro Kobayashi, Shigeo Ikegawa, Reiko Kiyonami

研究成果: Article査読

58 被引用数 (Scopus)

抄録

A new parallel liquid chromatography/tandem mass spectrometry (LC/MS/MS) system has been developed, in which the mass detector was shared between two staggered parallel chromatographic runs. Since the chromatography for biofluids assay generally requires good analyte retention and thus tends to leave large blank chromatographic windows, this parallel system allowed the efficient use of the mass detector during these blank windows, resulting in significantly improved sample throughput. Also, in order to remove the bottleneck in sample extraction for this parallel separation system, a high-flow extraction device was used to perform on-line extraction. This allowed for the direct injection of biofluids onto the system. The performance and capability of this system was evaluated in tests that contained a single analyte (oxazepam) and multiple analytes (12-in-1). The results indicated that the data generated from this system were comparable to those obtained on a conventional single-column system. An application of the system for high-throughput pharmacokinetic screening of drug candidates was also demonstrated.

本文言語English
ページ(範囲)73-81
ページ数9
ジャーナルRapid Communications in Mass Spectrometry
15
2
DOI
出版ステータスPublished - 2001 2月 3

ASJC Scopus subject areas

  • 分析化学
  • 分光学
  • 有機化学

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