TY - JOUR
T1 - The comprehensive analysis of microRNA in hearts of stress model rat
AU - Hashiyada, Masaki
AU - Hosoya, Tadashi
AU - Akane, Atsushi
AU - Matsumoto, Tomohiro
AU - Yoshimura, Sumitaka
AU - Tokiyasu, Takuma
AU - Funayama, Masato
N1 - Funding Information:
This work was supported by JSPS KAKENHI Grant Number 25460860.
Publisher Copyright:
© 2015 Elsevier Ireland Ltd
PY - 2015/12/1
Y1 - 2015/12/1
N2 - Non-coding microRNAs (miRNAs) hold enormous potential as a postmortem biomarker to quantify the physical stress applied before the death. Excited delirium syndrome causes a sudden unexpected death under agitated and excited situation. The forensic autopsies and laboratory tests can't reveal the mechanism of the sudden death. Then, we constructed a rat model of excited delirium syndrome-like situation by applying three stress modalities, alcohol intake for 3.8 g/kg body weight, treadmill running for 90 min, and suppression by 2 kg water bag for 90 min. The rats were divided into three groups: the control, the stress group A with alcohol intake and running, and the stress group B with alcohol intake, running and suppression. After the rats were euthanized by sevoflurane over-dose, total RNA was extracted from 20 mg left ventricle tissues. The small RNA library and templates were prepared for the next generation sequencer (NGS) analysis with Ion PGM system and Ion 318Chip (Thermo Fisher Scientific). The obtained data were analyzed by Torrent Suite software with reference to the rat miRNA databases. The top 30 miRNAs in expression level were selected and analyzed by statistical methods between the control and stress groups. The seven miRNAs (miR-126a-3p, miR-143-3p, miR-16-5p, miR-22-3p, miR-26a-5p, miR-29a-3p, and miR-29b-3p) showed the significant difference in expression level among the groups. Especially, miR-143-3p and miR-22-3p might reflect the physical stress on rat hearts well.
AB - Non-coding microRNAs (miRNAs) hold enormous potential as a postmortem biomarker to quantify the physical stress applied before the death. Excited delirium syndrome causes a sudden unexpected death under agitated and excited situation. The forensic autopsies and laboratory tests can't reveal the mechanism of the sudden death. Then, we constructed a rat model of excited delirium syndrome-like situation by applying three stress modalities, alcohol intake for 3.8 g/kg body weight, treadmill running for 90 min, and suppression by 2 kg water bag for 90 min. The rats were divided into three groups: the control, the stress group A with alcohol intake and running, and the stress group B with alcohol intake, running and suppression. After the rats were euthanized by sevoflurane over-dose, total RNA was extracted from 20 mg left ventricle tissues. The small RNA library and templates were prepared for the next generation sequencer (NGS) analysis with Ion PGM system and Ion 318Chip (Thermo Fisher Scientific). The obtained data were analyzed by Torrent Suite software with reference to the rat miRNA databases. The top 30 miRNAs in expression level were selected and analyzed by statistical methods between the control and stress groups. The seven miRNAs (miR-126a-3p, miR-143-3p, miR-16-5p, miR-22-3p, miR-26a-5p, miR-29a-3p, and miR-29b-3p) showed the significant difference in expression level among the groups. Especially, miR-143-3p and miR-22-3p might reflect the physical stress on rat hearts well.
KW - microRNA (miRNA)
KW - next generation sequencer (NGS)
KW - physical stress
KW - rat
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U2 - 10.1016/j.fsigss.2015.09.220
DO - 10.1016/j.fsigss.2015.09.220
M3 - Article
AN - SCOPUS:84951845880
VL - 5
SP - e557-e558
JO - Forensic Science International: Genetics Supplement Series
JF - Forensic Science International: Genetics Supplement Series
SN - 1875-1768
ER -