A tetrasaccharide consisting of D-galactose, N-acetylneuraminic acid, N-acetylglucosamine and N-acetylgalactosaminitol (1 : 1 : 1 : 1) was obtained from bovine colostrum κ-casein. It is the main carbohydrate portion of a caseinoglycopeptide formed by alkaline borohydride treatment. The carbohydrate sequence was analyzed by enzymatic and chemical methods and by mass spectrometry, and was shown to be N-acetylglucosaminyl-β-1-3-galactosyl-β-1-3-[N-acetylneuraminyl-α-2-6-]-N-acetylgalactosaminitol.
ASJC Scopus subject areas
- Molecular Biology