The Ca2+-ATPase of the Scallop Sarcoplasmic Reticulum Is of a Cold-adapted Type

D. Sato, T. Takahashi, G. Tajima, C. Sato, Y. Nagata, T. Yamamoto, J. Nakamura

研究成果: Article査読

抄録

At 0 to 20°C, the Ca2+-ATPase activity of the scallop sarcoplasmic reticulum (SR) was observed to be 7-60% of the peak activity at 30°C, while the ATPase activity of the rabbit SR was 0-7% of its peak at 55°C. The relative rabbit ATPase activity (0.7-7.0%) at 7-20°C became higher (6-15 times) and lower (1/4-1/2), respectively, by the solubilization of the rabbit ATPase with a detergent, dodecyloctaethylenglycol monoether, and by the reconstitution of the ATPase with asolectin (soybean lecithin). No activity at 0°C remained irrespective of these treatments. The relative scallop ATPase activity at 0-20°C was, however, scarcely affected by such solubilization and reconstitution. In contrast to the rabbit ATPase, the scallop ATPase seems to be capable of operating independently without the help of the membrane lipid at low temperature.

本文言語English
ページ(範囲)33-39
ページ数7
ジャーナルJournal of Membrane Biology
196
1
DOI
出版ステータスPublished - 2003 11 1

ASJC Scopus subject areas

  • 生物理学
  • 生理学
  • 細胞生物学

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