The amino-acid sequence of a lectin of the acorn barnacle Megabalanus rosa

Koji Muramoto, Hisao Kamiya

    研究成果: Article査読

    59 被引用数 (Scopus)

    抄録

    The complete amino-acid sequence of a lectin isolated from the coelomic fluid of the acorn barnacle Megabalanus rosa has been determined. The lectin (Mr 64 000) is composed of four identical subunits of 138 amino acids. The amino-acid sequence and the location of two interchain and three intrachain disulfide bridges of the subunit were determined by the manual sequencing of peptides derived from the protein by digestion with trypsin, chymotrypsin and Staphylococcus aureus V8 proteinase, as well as fragments produced by cleavage with cyanogen bromide. The Cys-Pro-Pro-Cys sequence at the interchain disulfide bridges was the same as that of the hinge region of human immunoglobulin IgG1. The amino acid sequence of M. rosa lectin includes some regions homologous to those in Sarcophaga (flesh fly) lectin.

    本文言語English
    ページ(範囲)285-295
    ページ数11
    ジャーナルBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
    874
    3
    DOI
    出版ステータスPublished - 1986 12 12

    ASJC Scopus subject areas

    • 生物理学
    • 構造生物学
    • 生化学
    • 分子生物学

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