The advantages of cDNA microarray as an effective tool for identification of reproductive organ-specific genes in a model legume, Lotus japonicus

Makoto Endo, Hitoshi Matsubara, Takao Kokubun, Hiromi Masuko, Yoshihito Takahata, Tohru Tsuchiya, Hiroo Fukuda, Taku Demura, Masao Watanabe

研究成果: Article査読

53 被引用数 (Scopus)

抄録

To understand the molecular mechanisms intrinsic to reproductive organ development a cDNA microarray, fabricated from flower bud cDNA clones, was used to isolate genes, which are specifically expressed during the development of the anther and pistil in Lotus japonicus. Cluster analysis of the microarray data revealed 21 and 111 independent cDNA groups, which were specifically expressed in immature and mature anthers, respectively. RT-PCR was performed to provide a direct assessment of the accuracy and reproducibility of our approach. Confirmation of our results suggests that cDNA microarray technology is an effective tool for identification of novel reproductive organ-specific genes.

本文言語English
ページ(範囲)229-237
ページ数9
ジャーナルFEBS Letters
514
2-3
DOI
出版ステータスPublished - 2002 3 13
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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