The β-1,3-exoglucanase gene exgA (exg1) of Aspergillus oryzae is required to catabolize extracellular glucan, and is induced in growth on a solid surface

Koichi Tamano, Yuki Satoh, Tomoko Ishii, Yasunobu Terabayashi, Shinsaku Ohtaki, Motoaki Sano, Tadashi Takahashi, Yasuji Koyama, Osamu Mizutani, Keietsu Abe, Masayuki Machida

    研究成果: Article査読

    34 被引用数 (Scopus)

    抄録

    The biological role of ExgA (Exg1), a secretory β-1,3-exoglucanase of Aspergillus oryzae, and the expression pattern of the exgA (exg1) gene were analyzed. The exgA disruptant and the exgA-overexpressing mutant were constructed, and phenotypes of both mutants were compared. Higher mycelial growth rate and conidiation efficiency were observed for the exgA-overexpressing mutant than for the exgA disruptant when β-1,3-glucan was supplied as sole carbon source. On the other hand, no difference in phenotype was observed between them in the presence or absence of the inhibitors of cell wall β-glucan remodeling when grown with glucose. exgA Expression was induced in growth on solid surfaces such as filter membrane and onion inner skin. A combination of poor nutrition and mycelial attachment to a hydrophobic solid surface appears to be an inducing factor for exgA expression. These data suggest that ExgA plays a role in β-glucan utilization, but is not much involved in cell wall β-glucan remodeling.

    本文言語English
    ページ(範囲)926-934
    ページ数9
    ジャーナルBioscience, Biotechnology and Biochemistry
    71
    4
    DOI
    出版ステータスPublished - 2007

    ASJC Scopus subject areas

    • バイオテクノロジー
    • 分析化学
    • 生化学
    • 応用微生物学とバイオテクノロジー
    • 分子生物学
    • 有機化学

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