The objective of the present paper was to develop a short-term testing method in vitro to measure anastomosis strength under controlled pulsatile pressure in a through flow system for the comparative evaluation of vascular graft materials, with respect to anastomosis. By this method, it is possible to estimate conveniently and effectively the damage process and the degradation in strength of the anastomosis part between a blood vessel and a vascular substitute. In this methodology, the double anastomosed technique was also proposed. One of the merits of this technique is that it reduces the variability of the experimental data caused by the differences of natural blood vessels due to individual variation. The study was carried out looking at the effect of pulsatile pressure amplitude, the number of pulsatile pressure cycles, and the difference of the compliance on the degradation of the strength of the anastomosis part between blood vessel and vascular substitute, with respect to time sequence. The effect of the pressure amplitude changing with respect to time sequence on the anastomosis strength was also studied. The damage at the anastomosed part was morphologically studied. The results obtained by these methods may be considered as relevant mechanically in vivo, except that the effect of tissue reorganization should also be taken into account.
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