Temperature-modulated adsorption of poly(N-isopropylacrylamide)-grafted ferritin on solid substrate

Yoshikazu Kumashiro, Yasuhiro Ikezoe, Tomohiro Hayashi, Youichi Okabayashi, Kaoru Tamada, Masayuki Yamato, Teruo Okano, Masahiko Hara

研究成果: Article査読

3 被引用数 (Scopus)


Ferritin grafted with temperature-responsive poly(N-isopropylacrylamide) (PIPAAm-ferritin) was synthesized by a coupling reaction using PIPAAm and ferritin for obtaining stimuli-responsive biomaterials. The hydrodynamic diameter of PIPAAm-ferritins in aqueous solution increased at 37°C at a higher protein concentration (>0.2. mg/mL) because of the intermolecular aggregation through the hydrophobic interaction of PIPAAm chains. On the other hand, PIPAAm-ferritins at a lower concentration (<0.2. mg/mL) were unable to increase their size even at 37°C. The adsorption kinetics of PIPAAm-ferritins on hydrophobically modified Si substrate were evaluated with a quartz crystal microbalance in 10. mmol/L Bis-Tris/HCl buffer (pH 5.8) with and without poly(oxyethylene) sorbitan monolaurate (TWEEN 20) (0.05. wt%) as a surfactant. Although the adsorption of PIPAAm-ferritins on hydrophobic Si substrate at 25°C in the buffer with TWEEN 20 was hardly observed, PIPAAm-ferritins were considerably adsorbed on the substrate at 37°C, indicating that the hydrophobic interaction between the substrate and PIPAAm grafts on the ferritins after the destruction of the hydrophobic interaction between the protein and the substrate by TWEEN 20.

ジャーナルColloids and Surfaces B: Biointerfaces
出版ステータスPublished - 2012 6月 15

ASJC Scopus subject areas

  • バイオテクノロジー
  • 表面および界面
  • 物理化学および理論化学
  • コロイド化学および表面化学


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