Temperature and salt concentration alter base-sequence selectivity of a duplex DNA-binding protein

Satoru Nagatoishi, Yoshikazu Tanaka, Motonori Kudou, Kouhei Tsumoto

研究成果: Article査読

1 被引用数 (Scopus)

抄録

A structural polymorphism of nucleic acids, which depends on the concentration of cations and the conditions of hydration, are strongly involved with interactions between DNA and proteins. In this paper, we report that different DNA sequences bound to hyperthermostable TATA-box-binding protein (PhoTBP) at different combinations of temperature and salt concentration in in vitro selection experiments. As a result of the interaction of-these selected DNAs with PhoTBP, characteristic changes in the numbers of water molecules and ions occurred under each condition of the selection experiment. This finding could help us to understand the solvent environment-dependent preference for base sequences in protein-DNA interactions.

本文言語English
ページ(範囲)98-101
ページ数4
ジャーナルMolecular BioSystems
6
1
DOI
出版ステータスPublished - 2009
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分子生物学

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