Technical communication Immunogold light and electron microscopic detection of amyloi plaques in transmissible spongiform encephalopathies

J. DOERR‐SCHOTT, T. KITAMOTO, J. TATEISHI, J. W. BOELLAARD, N. HELDT, C. LICHTE

研究成果: Article査読

12 被引用数 (Scopus)

抄録

The antigenicity of the ‘prion’ protein amyloid fibrils was shown to be preserved after glutaraldehyde/OsO4 fixation in uranyl acetate‐ stained brain tissue blocks from patients with Gerstmann‐Straussler syndrome (GSS) and from mice infected with Creutzfeldt‐Jakob disease (CJD). Amyloid plaques were demonstrated by light microscopy in immunogold silver‐intensified semithin sections. Under the electron microscope, the amyloid fibrils were labelled in immunogold‐reacted ultrathin sections using an antiserum prepared against GSS amyloid plaque cores and mouse amyloid fibrils respectively. The influence of various oxidizing agents (hydrogen peroxide, sodium metaperiodate) on the tissue preservation and the immunohistochemical detection was tested.

本文言語English
ページ(範囲)85-89
ページ数5
ジャーナルNeuropathology and Applied Neurobiology
16
1
DOI
出版ステータスPublished - 1990 2
外部発表はい

ASJC Scopus subject areas

  • 病理学および法医学
  • 組織学
  • 神経学
  • 臨床神経学
  • 生理学(医学)

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