Tauropine dehydrogenase from the starfish Asterina pectinifera (Echinodermata: Asteroidea): Presence of opine production pathway in a deuterostome invertebrate

Nobuhiro Kan-no, Minoru Sato, Takehiko Yokoyama, Eizoh Nagahisa, Yoshikazu Sato

研究成果: Article査読

5 被引用数 (Scopus)

抄録

Tauropine dehydrogenase (tauropine: NAD oxidoreductase; TaDH) was purified to homogeneity from the body wall of the starfish Asterina pectinifera Muller et Troschel (Echinodermata: Asteroidea) by means of (NH4)2SO4 precipitation followed by column chromatographies on DEAE-cellulose, Sephadex G75, Macro-prep ceramic hydroxyapatite, PBE 94, and Toyopearl HW50S. The enzyme was a monomeric protein of approximately 42 000 Da and pI 5.2. The maximum rate of the tauropine biosynthetic reaction was observed at pH 6.0, and that of the tauropine catabolic reaction was at pH 8.7-9.2. Taurine and pyruvate were the preferred substrates. The tauropine catabolic reaction was inhibited by the substrate tauropine: the peak rate was observed at 12.5 mM. Apparent K(m) values for NADH, taurine, and pyruvate were 0.036±0.002, 21.3±1.6, and 0.46±0.02 mM, respectively, and for tauropine and NAD+ were 2.64±0.73 and 0.068±0.005 mM, respectively. The molecular and catalytic properties of the starfish TaDH were basically similar to those of TaDH from other species belonging to the lower invertebrate phyla and the middle phyla of Protostomia. Tauropine accumulation in vivo during experimental anoxia was also demonstrated. These results gave clear evidence of opine production pathway in deutrostome invertebrate. Copyright (C) 1998 Elsevier Science Inc.

本文言語English
ページ(範囲)323-332
ページ数10
ジャーナルComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
121
3
DOI
出版ステータスPublished - 1998 1 1

ASJC Scopus subject areas

  • 生化学
  • 生理学
  • 分子生物学

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