Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry

Caterina Pettinella, Seon Hwa Lee, Francesco Cipollone, Ian A. Blair

研究成果: Article査読

61 被引用数 (Scopus)

抄録

The quantitative analysis of fatty acid composition in atherosclerotic plaques provides a way to monitor the underlying etiology of atherosclerosis. Previously, the method of choice for analyzing fatty acids in biological samples was gas chromatography/mass spectrometry (GC/MS); however, recent developments in electrospray ionization (ESI)/liquid chromatography (LC)/tandem mass spectrometry have made it a superior alternative. Previous research has largely focused on global analyses of intact lipids rather than more targeted analysis of the fatty acids themselves. We have now developed a targeted, stable isotope dilution LC-electrospray ionization/multiple reaction monitoring/MS method for the quantitative analysis of 10 fatty acids (myristic, palmitic, stearic, oleic, linoleic, α-linolenic, γ-linolenic, arachidonic, eicosapentaenoic, and docosahexaenoic acids) using their trimethylaminoethyl ester (TMAE) derivatives to improve sensitivity. The method was validated, had a detection limit in the fmol range, and was used in the analysis of fatty acids in atherosclerotic plaques from carotid arteries.

本文言語English
ページ(範囲)168-176
ページ数9
ジャーナルJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
850
1-2
DOI
出版ステータスPublished - 2007 5 1
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 臨床生化学
  • 細胞生物学

フィンガープリント

「Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル