Targeted expression of IP3 sponge and IP3 DsRNA impaires sugar taste sensation in Drosophila

Kazue Usui-Aoki, Ken Matsumoto, Masayuki Koganezawa, Sou Kohatsu, Kunio Isono, Hiroshi Matsubayashi, Masa Toshi Yamamoto, Ryu Ueda, Kuniaki Takahashi, Kaoru Saigo, Katsuhiko Mikoshiba, Daisuke Yamamoto

研究成果: Article査読

21 被引用数 (Scopus)

抄録

We evaluated the role of IP3 in sugar taste reception in Drosophila melanogaster by inactivating the IP3 signaling using genetic tools. We used the "IP3 sponge," composed of the modified ligand-binding domain from the mouse IP3 receptor, which was designed to absorb IP3 in competition with native IP3 receptors. Another tool was a transgene that generates double-stranded RNA against IP3 receptor mRNA. Both inhibitors diminished the sensitivity of flies to trehalose and sucrose, as estimated by behavioral assays and electrophysiological recordings from the sugar receptor cells. The result indicates that IP3 signaling is indispensable for sugar reception in Drosophila.

本文言語English
ページ(範囲)123-141
ページ数19
ジャーナルJournal of neurogenetics
19
3-4
DOI
出版ステータスPublished - 2005 7

ASJC Scopus subject areas

  • 遺伝学
  • 細胞および分子神経科学

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