Target-protein-selective inactivation and labelling using an oxidative catalyst

Shinichi Sato, Michihiko Tsushima, Hiroyuki Nakamura

研究成果: Review article査読

8 被引用数 (Scopus)

抄録

Reactive oxygen species (ROS) and radical species generated using oxidative single-electron transfer (SET) catalysts are highly reactive, inducing local environmental oxidative reactions, resulting in protein inactivation and labelling in proximity to the catalysts. Oxidative catalysts bound to the target protein generate ROS which induce oxidation only within a limited radius (∼30 nm), resulting in target-protein-selective inactivation. On the other hand, protein chemical labelling reactions via ROS or SET induced by the catalysts are completed in proximity to the catalyst. These proximity labelling techniques have recently attracted considerable attention as innovative tools to elucidate protein interaction mapping and unknown protein-protein interaction (PPI) partners. Not only can peroxidases be genetically introduced into the protein of interest but also ligand-conjugated catalysts can catalyze oxidative SET reactions in a protein mixture under intracellular conditions. In this review, we focus on two approaches of selective inactivation of protein functions and selective protein labelling using oxidative SET catalysts.

本文言語English
ページ(範囲)6168-6179
ページ数12
ジャーナルOrganic and Biomolecular Chemistry
16
34
DOI
出版ステータスPublished - 2018
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 物理化学および理論化学
  • 有機化学

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