Synthetic-biology-based discovery of a fungal macrolide from: Macrophomina phaseolina

Yohei Morishita, Terutaka Sonohara, Tohru Taniguchi, Kiyohiro Adachi, Makoto Fujita, Teigo Asai

研究成果: Article査読

6 被引用数 (Scopus)

抄録

A synthetic biology approach based on genome mining and heterologous biosynthesis is a powerful tool for discovering novel natural products from a tremendous gene resource. We carried out fungal genome mining guided by a polyketide synthase gene using a public database and found a putative macrolide biosynthetic gene cluster with a highly reducing polyketide synthase gene and a thioesterase gene in Macrophomina phaseolina. Reconstitution of the cluster in Aspergillus oryzae, a model heterologous host for fungal natural product biosynthesis, produced a new 12-membered macrolide, phaseolide A. The absolute stereochemistry was elucidated by vibrational circular dichroism spectroscopy and the crystalline sponge method.

本文言語English
ページ(範囲)2813-2816
ページ数4
ジャーナルOrganic and Biomolecular Chemistry
18
15
DOI
出版ステータスPublished - 2020 4 21

ASJC Scopus subject areas

  • 生化学
  • 物理化学および理論化学
  • 有機化学

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