Synthesis of Staudinger-type molecular probe for catch-and-release purification of the binding protein for potassium isolespedezate, a leaf-closing substance of leguminous plant

Tomohiko Fujii, Nobuki Kato, Izumi Iwakura, Yoshiyuki Manabe, Minoru Ueda

研究成果: Article

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We synthesized azide-containing photoaffinity probe 1 based on the structure of potassium isolespedezate. This probe can be used for catch-and-release-mechanism purification of binding protein for 1: photo-crosslinking with 1 gave azide-labeled receptor which can be captured by phosphane-linked gel matrix by the Staudinger ligation. After washing the gel, the caught binding protein can be released by the reductive cleavage of disulfide bond in 1. This process can be used as a convenient method for the purification of binding protein for bioactive natural product.

元の言語English
ページ(範囲)52-53
ページ数2
ジャーナルChemistry Letters
37
発行部数1
DOI
出版物ステータスPublished - 2008 1 5

ASJC Scopus subject areas

  • Chemistry(all)

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