Synthesis and in situ insertion of a site-specific fluorescently labeled membrane protein into cell-sized liposomes

Takuma Ohtsuka, Satoshi Neki, Tamotsu Kanai, Kazunari Akiyoshi, Shin Ichiro M. Nomura, Takashi Ohtsuki

研究成果: Article査読

14 被引用数 (Scopus)

抄録

The integral membrane protein bacteriorhodopsin, containing a fluorescent amino acid at a specific position, was synthesized in the presence of hydrated lipid films using an in vitro translation system expanded with a four-base codon/anticodon pair. Cell-sized liposomes with the labeled protein inserted into the liposome membranes were generated after the translation reaction. This study also demonstrated that this labeling method could be used to analyze the dynamic properties of membrane proteins in situ by fluorescence correlation spectroscopy.

本文言語English
ページ(範囲)97-101
ページ数5
ジャーナルAnalytical Biochemistry
418
1
DOI
出版ステータスPublished - 2011 11月 1
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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