Synthesis and evaluation of the luciferase-oligodeoxynucleotide for the sequence-selective detection of nucleic acids

Fumi Nagatsugi, Ryusuke Nakahara, Kaori Inoue, Shigeki Sasaki

研究成果: Article

6 引用 (Scopus)

抜粋

A new method for the synthesis of ODN-luciferase conjugate was investigated as a signal-amplifying sensor of the target nucleic acids. The conjugation of the luciferase was successfully achieved between the cysteine residue and the 2-amino-6-vinylpurine nucleoside of the ODN probe without significant inactivation of luciferase. The ODN-luciferase conjugate modified with PEG retained the luciferase activity and selectivity during the hybridization with the target ODN.

元の言語English
ページ(範囲)562-567
ページ数6
ジャーナルArchiv der Pharmazie
341
発行部数9
DOI
出版物ステータスPublished - 2008 9 1

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery

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