Synthesis and characterization of sapecin and sapecin B

Jae Il Kim, Hideo Iwai, Shoichiro Kurata, Masami Takahashi, Katuyoshi Masuda, Ichio Shimada, Shunji Natori, Yoji Arata, Kazuki Sato

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Two insect defencins, sapecin and sapecin B, were chemically synthesized to confirm their structure and antibacterial activity and also to examine the possibility that these peptides bind to the same site on the large conductance calcium-activated potassium channel as charybdotoxin. Both synthetic peptides showed the same antibacterial activity as native sapecins, indicating that the synthetic peptides folded correctly in the chemical synthesis. Synthetic sapecins did not show an inhibitory effect on [125I]charybdotoxin binding to rat brain synaptic membranes, suggesting that sapecin B recognizes a different binding site from that of charybdotoxin despite the similar structural motif.

本文言語English
ページ(範囲)189-192
ページ数4
ジャーナルFEBS Letters
342
2
DOI
出版ステータスPublished - 1994 4月 4
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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