Susceptibility of Plasmodium falciparum cyclic AMP-dependent protein kinase and its mammalian homologue to the inhibitors

Atsushi Sudo, Kentaro Kato, Kyousuke Kobayashi, Yukinobu Tohya, Hiroomi Akashi

研究成果: Article査読

23 被引用数 (Scopus)

抄録

Cyclic AMP-dependent protein kinase (protein kinase A, PKA) is a key element in many cell signaling pathways. An essential role of Plasmodium falciparum PKA (PfPKA) activity was reported in the intraerythrocytic growth of the malaria parasite. However, molecular characterization of PfPKA using purified recombinant proteins has not yet been performed. Here, we report the first successful purification of the enzymatically active PKA catalytic subunit of P. falciparum (PfPKA-C) using a wheat germ cell-free expression system. Interestingly, parasite enzymatic activity was weakly inhibited as compared with the inhibition of mammalian PKA catalytic subunit (PKA-C) by the specific PKA inhibitor, H89. Furthermore, PfPKA-C was only slightly inhibited by protein kinase inhibitor (PKI). These results suggest that substrate sites of PfPKA-C may be different from those of mammalian PKA-Cs. In addition, potential PKI corresponding to malarial PKA-C would also be different from those of mammalian cells.

本文言語English
ページ(範囲)138-142
ページ数5
ジャーナルMolecular and Biochemical Parasitology
160
2
DOI
出版ステータスPublished - 2008 8
外部発表はい

ASJC Scopus subject areas

  • 寄生虫科
  • 分子生物学

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