Suppression of cytokine-induced expression of adrenomedullin and endothelin-1 by dexamethasone in T98G human glioblastoma cells

Kazuhiro Takahashi, Reiko Udono-Fujimori, Kazuhito Totsune, Osamu Murakami, Shigeki Shibahara

研究成果: Article査読

14 被引用数 (Scopus)

抄録

There is accumulating evidence showing that glial cells and gliomas secrete some neuropeptides and vasoactive peptides, such as adrenomedullin and endothelin-1. We have previously shown that expression of these two peptides is induced by inflammatory cytokines in T98G human glioblastoma cells. Glucocorticoids are frequently used for the treatment of inflammatory diseases and glioblastomas. We therefore studied effects of dexamethasone on expression of adrenomedullin and endothelin-1 in T98G human glioblastoma cells. Dexamethasone dose-dependently increased adrenomedullin mRNA levels and immunoreactive-adrenomedullin levels in the medium in T98G cells, whereas it decreased immunoreactive-endothelin levels in the medium. A combination of three cytokines, interferon-γ (100U/ml), tumor necrosis factor-α (20ng/ml) and interleukin-1β (10ng/ml) induced expression of adrenomedullin and endothelin-1 in T98G cells. Dexamethasone (10 -8mol/l) suppressed increases in expression of both adrenomedullin and endothelin-1 induced by these three cytokines. Thus, dexamethasone alone increased adrenomedullin expression whereas it suppressed the cytokine-induced expression of adrenomedullin in T98G cells. These findings raised the possibility that effects of dexamethasone on brain inflammation and glioblastomas may be partly mediated or modulated by its effects on expression of adrenomedullin and endothelin-1.

本文言語English
ページ(範囲)1053-1062
ページ数10
ジャーナルPeptides
24
7
DOI
出版ステータスPublished - 2003 7 1

ASJC Scopus subject areas

  • 生化学
  • 生理学
  • 内分泌学
  • 細胞および分子神経科学

フィンガープリント

「Suppression of cytokine-induced expression of adrenomedullin and endothelin-1 by dexamethasone in T98G human glioblastoma cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル