Superoxide generation mediated by 8-nitroguanosine, a highly redox-active nucleic acid derivative

Tomohiro Sawa, Takaaki Akaike, Koji Ichimori, Teruo Akuta, Kazuyoshi Kaneko, Hitoshi Nakayama, Dennis J. Stuehr, Hiroshi Maeda

研究成果: Article査読

50 被引用数 (Scopus)

抄録

Formation of 8-nitroguanosine may be characterized as nucleic acid modification induced by nitric oxide (NO). We show here that 8-nitroguanosine is a highly redox-active nucleic acid derivative that strongly stimulated superoxide generation from various NADPH-dependent reductases, including cytochrome P450 reductase and all isoforms of NO synthase. This reaction involves these reductases in a redox cycling reaction via single-electron reduction of 8-nitroguanosine to form 8-nitroguanosine anion radical. One electron is then transferred from this radical to molecular oxygen. 8-Nitroguanosine formed in vivo may function as a potent redox cofactor that intensifies oxyradical generation by various NADPH/reductase-like enzymes and thus participates in diverse physiological and pathological events.

本文言語English
ページ(範囲)300-306
ページ数7
ジャーナルBiochemical and biophysical research communications
311
2
DOI
出版ステータスPublished - 2003 11 14
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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