Study of the acute cardiovascular effects of several antihypertensive agents with the measurement of plasma catecholamines in mice

Makoto Tsunoda, Masanori Yamagishi, Kazuhiro Imai, Teruyuki Yanagisawa

研究成果: Article査読

11 被引用数 (Scopus)

抄録

A highly sensitive determination method was established for catecholamines (norepinephrine (NE), epinephrine, and dopamine) with high-performance liquid chromatography-peroxyoxalate chemiluminescence reaction detection. In this study, the method was applied to mouse plasma, and it was determined that only 10 μl of mouse plasma was necessary for the selective and reproducible determination of catecholamines. Studies were then conducted in acute cardiovascular effects of sodium nitroprusside, nicardipine, captopril (angiotensin-converting enzyme (ACE) inhibitor), candesartan, and olmesartan (type 1 angiotensin receptor antagonists (AT1 antagonists)) by this method. Sodium nitroprusside and nicardipine elevated plasma NE concentrations significantly, whereas the ACE inhibitor and the AT1 antagonists did not change plasma NE concentrations in anesthetized mice. These results suggested that angiotensin II-induced augmentation may be mainly carried through the central baroreflex pathway.

本文言語English
ページ(範囲)947-952
ページ数6
ジャーナルAnalytical and Bioanalytical Chemistry
394
4
DOI
出版ステータスPublished - 2009 6月

ASJC Scopus subject areas

  • 分析化学
  • 生化学

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