Structure determination of tubular crystals of membrane proteins. II. Averaging of tubular crystals of different helical classes

Koji Yonekura, Chikashi Toyoshima

研究成果: Article査読

16 被引用数 (Scopus)

抄録

A set of programs has been developed for averaging the data from tubular crystals belonging to different helical classes. This was done either by (i) cutting out molecules constituting a unit cell from density maps, and aligning and averaging them in real space; (ii) transforming the densities in a unit cell to layer-line data according to a (possibly artificial) helical symmetry, aligning and averaging them in reciprocal space. These methods were applied to tubular crystals of Ca2+-ATPase. Either method worked well and substantially improved the data quality. Transforming the reconstructed images to the layer-line data has many advantages and is essential for fully exploiting the power of averaging. Copyright (C) 2000 Elsevier Science B.V.

本文言語English
ページ(範囲)15-28
ページ数14
ジャーナルUltramicroscopy
84
1-2
DOI
出版ステータスPublished - 2000 7
外部発表はい

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 原子分子物理学および光学
  • 器械工学

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