Structural studies of a novel type of pentaantennary large glycan unit in the fertilization-associated carbohydrate-rich glycopeptide isolated from the fertilized eggs of Oryzias latipes

Tomohiko Taguchi, Akira Seko, Ken Kitajima, Yutaka Muto, Sadako Inoue, Kay Hooi Khoo, Howard R. Morris, Anne Dell, Yasuo Inoue

研究成果: Article査読

41 被引用数 (Scopus)

抄録

In a previous report (Kitajima, K., Inoue, S., and Inoue, Y. (1989) Dev. Biol. 132, 544-553), we found the presence of a heavily glycosylated polyprotein, 'H-hyosophorin,' isolated from the unfertilized eggs of Oryzias latipes. We now report our detailed analysis of the structure of the N- glycan chain in L-hyosophorin, the smallest repeating unit of H-hyosophorin, which was isolated from the fertilized eggs of O. latipes and formed from H- hyosophorin upon fertilization. The N-glycan structures were defined by a combination of compositional analysis, methylation analysis, selective chemical degradation (i.e. mild methanolysis, periodate-Smith degradation, and hydrazinolysis-nitrous acid deamination), enzymatic (endo-β- galactosidase, peptide:N-glycanase, and Newcastle disease virus sialidase) digestion, and instrumental analyses (one- and two-dimensional proton nuclear magnetic resonance spectroscopy and fast atom bombardment mass spectrometry) which revealed novel and unique features: (a) the presence of highly branched poly-N-acetyllactosamino pentaantennary structures; (b) the presence of a β- galactosylated Lewis X antigenic epitope, Galβ1→4 Galβ1→4 (Fucα1→3)GlcNAcβ1→; (c) the presence of a β-galactosylated sialyl Lewis X structure, Galβ1→4(Neu5Acα2→3)Galβ1→4(Fucα1→3)GlcNAcβ1→; (d) the presence of Galβ1→4Galβ1→ and Galβ1→ 4Galβ1→4Galβ1→ as the major and minor groupings, respectively; and (e) the presence of the branched Gal residues, →4GlcNAcβ1→3(Galβ1→4) Galβ1→. This study represents the first detailed investigation regarding the nature of highly branched complex asparagine-linked pentaantennary glycans in glycoproteins. The unique expression of such bulky multiantennary glycan units on proteins could be essential during early embryogenesis.

本文言語English
ページ(範囲)8762-8771
ページ数10
ジャーナルJournal of Biological Chemistry
269
12
出版ステータスPublished - 1994 3 25
外部発表はい

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

フィンガープリント 「Structural studies of a novel type of pentaantennary large glycan unit in the fertilization-associated carbohydrate-rich glycopeptide isolated from the fertilized eggs of Oryzias latipes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル