Structural optimization analysis of endothelial cell remodeling to fluid flow

T. Ohashi, S. Seo, T. Matsumoto, Masaaki Sato

研究成果: Conference article査読

1 被引用数 (Scopus)


A finite element analysis using structural optimization method was performed to simulate the remodeling of bovine aortic endothelial cells (BAECs). BAECs showed marked elongation and aligned in the flow direction after exposing to shear stress of 2 Pa for 24 hours. An atomic force microscope (AFM) was used to measure cell surface geometries, showing that the peak cell height decreased significantly from 2.8 ± 1.0 μm (mean ± SD) to 1.4 ± 0.5 μm with fluid flow. The fluorescence images showed that control cells exhibited dense peripheral bands of F-actin filaments, while sheared cells exhibited centrally located F-actin stress fibers parallel to the flow direction. A finite element model was generated on the basis of the cell surface geometries, in which elastic modulus of each element was changed in accordance with an objective stress together with update of cell shape. The results showed that the cell height decreased with fluid flow and the higher elastic modulus appeared in the upstream region of the nucleus in the final step, which may correspond with cytoskeletal structure. The present analysis should be effective for clarifying the remodeling of endothelial cells.

ジャーナルAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
出版ステータスPublished - 2002 1月 1
イベントProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
継続期間: 2002 10月 232002 10月 26

ASJC Scopus subject areas

  • 信号処理
  • 生体医工学
  • コンピュータ ビジョンおよびパターン認識
  • 健康情報学


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