Structural feature of the major but not cytokine-inducing molecular species of lipoteichoic acid

Masahito Hashimoto, Jun Ichi Yasuoka, Yasuo Suda, Haruhiko Takada, Takeshi Yoshida, Shozo Kotani, Shoichi Kusumoto

研究成果: Article査読

24 被引用数 (Scopus)

抄録

Previously, lipoteichoic acid (LTA) of Enterococcus hirae was found to exhibit definite cytokine-inducing activity but synthetic specimens which share the fundamental structural principles proposed for LTA had no corresponding activity. We also showed recently that several minor components totally less than 5% of the LTA fraction from E. hirae ATCC 9790 possessed the activity, whereas the major component (over 90%) did not. In the present study, the structure of the major component of LTA was studied in an attempt to elucidate the reason for the lack of the activity in the synthetic compounds. The major component of the LTA was first digested by hydrofluoric acid hydrolysis to cleave phosphodiester linkages present. The hydrolysis products were separated and characterized by means of NMR and MS. The linkage positions of the original phosphodiesters were determined from the NMR spectra of an alkali-treated product without hydrofluoric acid degradation. The compound was proved to consist of 1,3-linked poly(glycerophosphate) and a lipid anchor, Glc(α1-2)Glc(α1-3)acyl2Gro, the former being linked to the 6-position of the distal glucose of the latter. The 2-position of the glycerol residues in the glycerophosphate part were substituted by oligoglucose esterified partially with alanine. The gross structure elucidated here thus coincides with the previous conclusion described by Fischer. Thus, the molecular species with this so-called 'LTA structure' is not responsible for the cytokine-inducing activity.

本文言語English
ページ(範囲)779-786
ページ数8
ジャーナルJournal of biochemistry
121
4
DOI
出版ステータスPublished - 1997

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

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