Structural dynamics of cereal mitochondrial genomes as revealed by complete nucleotide sequencing of the wheat mitochondrial genome

Yasunari Ogihara, Yukiko Yamazaki, Koji Murai, Akira Kanno, Toru Terachi, Takashi Shiina, Naohiko Miyashita, Shuhei Nasuda, Chiharu Nakamura, Naoki Mori, Shigeo Takumi, Minoru Murata, Satoshi Futo, Koichiro Tsunewaki

研究成果: Article査読

145 被引用数 (Scopus)

抄録

The application of a new gene-based strategy for sequencing the wheat mitochondrial genome shows its structure to be a 452 528 bp circular molecule, and provides nucleotide-level evidence of intra-molecular recombination. Single, reciprocal and double recombinant products, and the nucleotide sequences of the repeats that mediate their formation have been identified. The genome has 55 genes with exons, including 35 protein-coding, 3 rRNA and 17 tRNA genes. Nucleotide sequences of seven wheat genes have been determined here for the first time. Nine genes have an exon-intron structure. Gene amplification responsible for the production of multicopy mitochondrial genes, in general, is species-specific, suggesting the recent origin of these genes. About 16, 17, 15, 3.0 and 0.2% of wheat mitochondrial DNA (mtDNA) may be of genic (including introns), open reading frame, repetitive sequence, chloroplast and retro-element origin, respectively. The gene order of the wheat mitochondrial gene map shows little synteny to the rice and maize maps, indicative that thorough gene shuffling occurred during speciation. Almost all unique mtDNA sequences of wheat, as compared with rice and maize mtDNAs, are redundant DNA. Features of the gene-based strategy are discussed, and a mechanistic model of mitochondrial gene amplification is proposed.

本文言語English
ページ(範囲)6235-6250
ページ数16
ジャーナルNucleic acids research
33
19
DOI
出版ステータスPublished - 2005

ASJC Scopus subject areas

  • 遺伝学

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