抄録
The structure of the N-linked carbohydrate chains of peptide isomerase from the venom of the funnel web spider (Agelenopsis aperta) has been analyzed. Carbohydrates were released from peptide isomerase by hydrazinolysis and reductively aminated with 2-aminopyridine. The fluorescent derivatives were purified by phenol/chloroform extraction, followed by size-exclusion HPLC. The structure of the purified pyridylamino (PA-) carbohydrate chains were analyzed by a combination of two-dimensional HPLC mapping, sugar composition analysis, sequential exoglycosidase digestions, and mass spectrometry. The peptide isomerase contains six kinds of N-linked carbohydrate chains of truncated high-mannose type, with a fucose α1-6 linked to the reducing N-acetylglucosamine in approximately 80% of them.
| 本文言語 | English |
|---|---|
| ページ（範囲） | 1211-1215 |
| ページ数 | 5 |
| ジャーナル | Bioscience, Biotechnology and Biochemistry |
| 巻 | 62 |
| 号 | 6 |
| DOI | |
| 出版ステータス | Published - 1998 1月 1 |
| 外部発表 | はい |
ASJC Scopus subject areas
- バイオテクノロジー
- 分析化学
- 生化学
- 応用微生物学とバイオテクノロジー
- 分子生物学
- 有機化学