Stoichiometric growth model for riboflavin-producing Bacillus subtilis

Toshiaki Mori, Shintaro Kishimoto, Kuniharu Ijiro, Atsushi Kobayashi, Yoshio Okahata

研究成果: Article査読

105 被引用数 (Scopus)

抄録

Rate equations for measured extracellular rates and macromolecular composition data were combined with a stoichiometric model to describe riboflavin production with an industrial Bacillus subtilis strain using errors in variables regression analysis. On the basis of this combined stoichiometric growth model, we explored the topological features of the B. subtilis metabolic reaction network that was assembled from a large amount of literature. More specifically, we simulated maximum theoretical yields of biomass and riboflavin, including the associated flux regimes. Based on the developed model, the importance of experimental data on building block requirements for maximum yield and flux calculations were investigated. These analyses clearly show that verification of macromolecular composition data is important for optimum flux calculations.

本文言語English
ページ(範囲)132-143
ページ数12
ジャーナルBiotechnology and Bioengineering
76
2
DOI
出版ステータスPublished - 2001 1月 1

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

フィンガープリント

「Stoichiometric growth model for riboflavin-producing Bacillus subtilis」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル