Stimulation of the phage λ pL promoter by integration host factor requires the carboxy terminus of the α-subunit of RNA polymerase

Hilla Giladi, Kazuhiko Igarashi, Akira Ishihama, Amos B. Oppenheim

研究成果: Article査読

37 被引用数 (Scopus)

抄録

Escherichia coli integration host factor (IHF) binds with high affinity to two tandem IHF consensus sequences located upstream from the pL promoter of bacteriophage lambda. IHF was shown to stimulate transcription initiation from the pL promoter by increasing close complex formation (KB). We show here, by the use of reconstituted mutant RNA polymerases, that the C-terminal portion of the α subunit of RNA polymerase plays an essential role in the stimulation of transcription by IHF. Our results are in agreement with the hypothesis that IHF, like the cAMP-CRP activator, increases the affinity of RNA polymerase to the promoter by protein-protein interaction.

本文言語English
ページ(範囲)985-990
ページ数6
ジャーナルJournal of Molecular Biology
227
4
DOI
出版ステータスPublished - 1992 10 20
外部発表はい

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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