Stimulation of muscarinic receptor in hippocampal neuron induces characteristic increase in cytosolic free Ca2+ concentration

Yoshihisa Kudo, Akihiko Ogura, Toshio Iijima

    研究成果: Article査読

    35 被引用数 (Scopus)

    抄録

    Changes in cytosolic free Ca2+ concentration ([Ca2+]i) in response to acetylcholine (ACh) were examined by fura-2 fluorometry in cultured rat hippocampal neurons. ACh (≧10-5 M) induced an increase in [Ca2+]i composed of fast transient and slow long-lasting phases. Atropine (10-8 M) abolished the fast component and greatly reduced the slow component. The slow component was selectively blocked by pirenzepine (10-6 M). The effect of ACh remained partially in a Ca2+-deficient medium where effects of l-glutamate and KCl (50 mM) were abolished. Present results suggest that ACh elevates [Ca2+]i by activation of muscarinic receptor subtypes, one of which is coupled with ion channels and the other of which transduces the ACh binding to mobilization of intracellularly stored Ca2+.

    本文言語English
    ページ(範囲)345-350
    ページ数6
    ジャーナルNeuroscience Letters
    85
    3
    DOI
    出版ステータスPublished - 1988 3 10

    ASJC Scopus subject areas

    • 神経科学(全般)

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