STED microscopy – super-resolution bio-imaging utilizing a stimulated emission depletion

Kohei Otomo, Terumasa Hibi, Yuichi Kozawa, Tomomi Nemoto

研究成果: Review article査読

16 被引用数 (Scopus)

抄録

One of the most popular super-resolution microscopies that breaks the diffraction barrier is stimulated emission depletion (STED) microscopy. As the optical set-up of STED microscopy is based on a laser scanning microscopy (LSM) system, it potentially has several merits of LSM like confocal or two-photon excitation LSM. In this article, we first describe the principles of STED microscopy and then describe the features of our newly developed two-photon excitation STED microscopy. On the basis of our recent results and those of other researchers, we conclude by discussing future research and new technologies in this field.

本文言語English
ページ(範囲)227-236
ページ数10
ジャーナルMicroscopy
64
4
DOI
出版ステータスPublished - 2015 8 1

ASJC Scopus subject areas

  • 構造生物学
  • 器械工学
  • 放射線学、核医学およびイメージング

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