SREC-II, a new member of the scavenger receptor type F family, trans-interacts with SREC-I through its extracellular domain

Junko Ishii, Hideki Adachi, Junken Aoki, Hiroyuki Koizumi, Susumu Tomita, Toshiharu Suzuki, Masafumi Tsujimoto, Keizo Inoue, Hiroyuki Arai

研究成果: Article査読

60 被引用数 (Scopus)

抄録

The scavenger receptor expressed by endothelial cells (SREC) with an extremely large cytoplasmic domain, was originally identified in a human endothelial cell line. In this study, we have cloned a second isoform named SREC-II and shown that there is a heterophilic interaction between SREC-I and -II at their extracellular domains. The cDNA for murine SREC-II encodes an 834-amino acid protein with 35% homology to SREC-I. Similar to SREC-I, SREC-II contains multiple epidermal growth factor-like repeats in its extracellular domain. However, in contrast to SREC-I, SREC-II had little activity to internalize modified low density lipoproteins (LDL). A Northern blot analysis revealed a tissue expression pattern of SREC-II similar to that of SREC-I with predominant expression in human heart, lung, ovary, and placenta. Mouse fibroblast L cells with no tendency to associate showed noticeable aggregation when SREC-I was overexpressed in these cells, whereas overexpression of SREC-II caused only slight aggregation. Remarkably, intense aggregation was observed when SREC-I-expressing cells were mixed with those expressing SREC-II. Deletion of almost all of the cytoplasmic receptor domain had no effect on the receptor expression and cell aggregation, indicating that solely the extracellular domain is involved in cell aggregation. The association of SREC-I and -II was effectively suppressed by the presence of scavenger receptor ligands such as acetylated LDL and oxidized LDL. These findings suggest that SREC-I and -II show weak cell-cell interaction by their extracellular domains (termed homophilic trans-interaction) but display strong heterophilic transinteraction through the extracellular epidermal growth factor-like repeat domains.

本文言語English
ページ(範囲)39696-39702
ページ数7
ジャーナルJournal of Biological Chemistry
277
42
DOI
出版ステータスPublished - 2002 10 18

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

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