Specificity of Traction Forces to Extracellular Matrix in Smooth Muscle Cells

T. Ohashi, H. Ichihara, N. Sakamoto, Masaaki Sato

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

Adherent cells are known to exert traction forces to adhere to their extracellular matrix at focal adhesions, where integrins interact with the extracellular matrix and mediate various cellular signals. Therefore, it is hypothesized that extracellular matrix-mediated integrin expression may modulate cellular traction forces, resulted in alterations in cell physiology. In this study, to test the hypothesis, traction force measurements are performed on bovine thoracic smooth muscle cells using a polydimethylsiloxane-based micropillar substrate coated with different types of extracellular matrix. The top of the micropillars was selectively coated with fibronectin (FN), vitronectin (VN), and type I collagen (COL I) at 30 and 50 μg/ml and cells were plated on the extracellular matrix-coated micropillars. For FN and VN, the cells have several spikes at the cell periphery, whereas, for COL I, the cell expresses relatively oval shape. At 30 μg/ml, traction forces were 5.5 ± 0.6, 5.8 ± 1.3 and 8.5 ± 2.2 nN for FN, VN, COL I, respectively. At 50 μg/ml, traction forces significantly increased compared to 30 μg/ml and were 12.4 ± 6.8, 13.5 ± 4.0 and 18.1± 4.7 nN for FN, VN, COL I, respectively. These results indicate that extracellular matrix may specifically modulate integrin expression followed by development of actin stress fibers, possibly leading to changes in traction forces.

本文言語English
ホスト出版物のタイトル13th International Conference on Biomedical Engineering - ICBME 2008
ページ2192-2193
ページ数2
DOI
出版ステータスPublished - 2009 12月 1
イベント13th International Conference on Biomedical Engineering, ICBME 2008 - , Singapore
継続期間: 2008 12月 32008 12月 6

出版物シリーズ

名前IFMBE Proceedings
23
ISSN(印刷版)1680-0737

Other

Other13th International Conference on Biomedical Engineering, ICBME 2008
国/地域Singapore
Period08/12/308/12/6

ASJC Scopus subject areas

  • バイオエンジニアリング
  • 生体医工学

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