Specificity of carbohydrate structures of gangliosides in the activity to regenerate the rat axotomized hypoglossal nerve

Michi Ichiro Itoh, Satoshi Fukumoto, Tsutomu Iwamoto, Akio Mizuno, Atsushi Rokutanda, Hide Ki Ishida, Makoto Kiso, Koichi Furukawa

研究成果: Article

15 引用 (Scopus)

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We previously reported that a ganglioside mixture from bovine brain could prevent neuronal death and promote regeneration in rats with hypoglossal nerve resection. In the present study, we have compared the neurotrophic effects of various glycosphingolipids including lactosylceramide. The findings revealed that GT1b had the activity of neuronal death prevention equivalent to a ganglioside mixture or autograft, while other glycolipids exhibited about 60% activity. However, the capability to promote the regeneration varied among glycolipids, that is, GT1b (86%), GD1b (55%), GD1a (35%), GQ1b (34%), GM1 (20%), lactosyl-ceramide (17%) in the number of horseradish peroxidase-positive neurons as an indicator of regeneration. The experiments with oligosaccharides of GT1b or GD1b and ceramide showed that the carbohydrate moiety mainly exerts neurotrophic effects. These findings suggested that fine structures of carbohydrate moiety in gangliosides are critical in the regenerative activity in this hypoglossal nerve regeneration system.

元の言語English
ページ(範囲)125-130
ページ数6
ジャーナルGlycobiology
11
発行部数2
DOI
出版物ステータスPublished - 2001 1 1
外部発表Yes

ASJC Scopus subject areas

  • Biochemistry

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